Order Guideline

Note :
1. Primer Walking - Single Strand Sequencing: 1µg/1 kb insert. If insert size is longer than 4kb, clone is required in an agar stab culture status.
2. If you have a blood and tissue of insect/plant/animal or something, please contact us by email. info@macrogen.com

a) Sample for individual tube :

- General glycerol stock/ Agar-stab/Agar-plate culture at room temperature. - 1.5mL Microcentrifuge Tube is recommended in a dried (lyophilized) form or solution ..(Nuclease-free TE or distilled water) at room temperature.. - Free re-sequencing is included

b) 96 Well Plate :

- 8 strip cap is recommended in a dried (lyophilized) form or solution (Nuclease-free TE or distilled water) .. at room temperature. - Re-sequencing is additionally charged. ** In case of Multi primers for one plate ** Only two custom primers (or two universal primers) in tubes could be acceptable for applying this Plate sequencing. Note : For three or more primers, another paired primer plate with your sample plate should be provided. If not, your order will be regarded as Normal sequencing reactions.

c) Primer :

- Primer : 10 pmol/uL in 20µl based on 5 samples - Primer Tm is suitable for service : 55°C ~ 60°C - Commonly used Universal primers are available for free of charge (ex. M13F, T7, T3) - Design and synthesize primers if you request. (US $0.60/base)

d) To apply Plate sequencing :

- 1 primer/1plate service : - Multi primers/ 1plate service :   Only two custom primers (or two universal primers) in tubes could be acceptable for applying this Plate   sequencing. Note : For three or more primers, another paired primer plate with your sample plate should be provided. If not, your order will be regarded as Normal sequencing reactions.