Next-Gen Sequencing

 

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NGS Sample Submission Guide
Sample Preparation
Start by checking requested input amount from our QC criteria.
DNA:
• Quantify your sample using fluorescent based method.
• Check quality of your sample by gel electrophoresis.
• Aliquot your DNA sample in nuclease free water or TE buffer in a clearly labeled 1.5~2.0ml microcentrifuge tube sealed with parafilm tightly. Refer to our packing guideline.
RNA:
• Quantify your sample using fluorescent based method.
• Check quality of your sample by BioAnalyzer or equivalent.
• Optimal condition for RNA storage and/or delivery is ethanol precipitation method (stable for 1 year at -20C) Alternatively, RNA transport reagent from various commercial companies can be used.
e.g. Ethanol Precipitation
1. All solutions should be nuclease-free or DEPC-treated water.
2. Add 0.1 volume of 3 M Sodium Acetate (NaOAc, pH5.5) to RNA solution and mix gently.
3. Add 2 volume of 100% ethanol to RNA solution and mix gently. If you have 100㎕ of RNA solution, add 10㎕ of 3 M Sodium Acetate (NaOAc, pH5.5) and 220㎕ of 100% ethanol to RNA solution and mix well gently.
• Aliquot your RNA sample in nuclease free water in a clearly labeled 1.5~2.0ml microcentrifuge tube sealed with parafilm tightly. Refer to our packing guideline. If dry ice shipping is needed, please contact your Macrogen representative.
Include a brief description of extraction and/or enrichment protocol used in the “Comments” fields of the order sheet.
Certain carriers and pellet paint types are not compatible with aspects of our sequencing technology.
Non-robust results have been observed from samples where Lithium Chloride (LiCl), fluorescent pellet paint, or tRNA was used prior to precipitation and resuspension for sample preparation input.
Shipping Samples to Macrogen
Place sealed individual microcentrifuge tubes in a 50ml disposable screw cap tube for additional insulation during shipment. To prevent sample tubes from moving during shipment, pack any remaining space in the 50ml tube with clean tissue paper prior to sealing. Refer to our packing guideline.
Contact your Macrogen representative to get access to our online shipment system. Following should be included in the package.
• Prepared sample tubes
• Adequate cooling agent e.g. ice pack, dry ice
• Cushioning material e.g. bubble wrap, foam, tissue paper
• Printed Macrogen order sheet
Print the 3x shipping labels and 3x commercial invoices, sign and enclose with your shipment and contact your local office of chosen courier to schedule a pick-up and dispatch for the same day.
Your sample and data will be discarded after 3 months of data delivery. You may extend the period with a special request.