Next-Gen Sequencing

 

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Single Cell RNA Sequencing
Introduction
If traditional transcriptome sequencing (also called bulk RNA sequencing) is the way to see the average gene expression in different cells, then Signal Cell RNA Sequencing can analyze thousands of transcription bodies simultaneously at a single cell level, so we can see the difference in gene expression values for different cell types. In addition, a full-length (clonotype level) analysis of the B cell, T cell is possible.
Library Preparation Kit
• 10X Genomics Chromium 5'GEX
• 10X Genomics Chromium 3'GEX
• 10X Genomics Chromium V(D)J
• 10X Genomics Chromium ATAC
Sequencing Platforms
• Illumina NovaSeq 6000 / HiSeq 4000 / HiSeq 2500
Data Analysis
Single Cell RNA Sequencing
Standard Data Analysis
• Gene expression and cell clustering analysis
• Simultaneous single cell analysis of gene expression
• Immune profiling and cell surface protein expression
Advanced Data Analysis
• Sample aggregation
• Gene expression and cell clustering analysis using Seurat
• Cell identification
• Differentially Expressed Genes (DEGs) analysis
• Gene ontology analysis
• Trajectory analysis
Single Cell Immune Profiling
• Paired full length V(D)J gene expression identification
• Clonality and diversity of TCR and/or BCR
Single Cell Surface Protein Profiling
• Surface protein expression and cell clustering analysis
Single Cell ATAC Sequencing
• Chromatin accessibility profiles and cell clustering analysis
Single Cell CNV Profiling
• Chromatin accessibility profiles and cell clustering analysis
*Data Analysis may vary depending on the availability of reference and the type of platform. For any inquiry of NGS service, please feel free to contact us.
E-mail : ngs@macrogen.eu